Bactericidal effect of S-nitrosothiols against clinical isolates from keratitis

نویسندگان

  • Angelino Julio Cariello
  • Paulo José Martins Bispo
  • Gabriela Freitas Pereira de Souza
  • Antonio Carlos Campos Pignatari
  • Marcelo Ganzarolli de Oliveira
  • Ana Luisa Hofling-Lima
چکیده

BACKGROUND The purpose of this study was to evaluate the antimicrobial activity of two nitric oxide donors, ie, S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylcysteine (SNAC), against clinical isolates from patients with infectious keratitis. METHODS Reference broth microdilution assays were performed to determine the minimum inhibitory and bactericidal concentrations for GSNO and SNAC against four American Type Culture Collection strains and 52 clinical isolates from patients with infectious keratitis as follows: 14 (26.9%) Pseudomonas species; 13 (25.0%) coagulase-negative Staphylococci; 10 (19.2%) Staphylococcus aureus; nine (17.3%) Serratia marcescens; and six (11.5%) Enterobacter aerogenes. Sterility control and bacterial growth control were also performed. RESULTS SNAC showed lower minimum inhibitory and bactericidal concentrations than GSNO for all clinical isolates from patients with infectious keratitis. For Gram-positive bacteria, mean minimum inhibitory and bactericidal concentrations were 2.1 ± 1.3 and 8.6 ± 3.8 mM for SNAC and 4.6 ± 3.2 and 21.5 ± 12.5 mM for GSNO (P < 0.01). For Gram-negative bacteria, mean minimum inhibitory and bactericidal concentrations were 3.3 ± 1.4 and 6.1 ± 3.4 mM for SNAC and 12.4 ± 5.4 and 26.5 ± 10.1 mM for GSNO (P < 0.01). The minimum bactericidal to inhibitory concentration ratio was ≤8 in 100% of all isolates tested for SNAC and in 94.2% tested for GSNO. CONCLUSIONS SNAC and GSNO had effective inhibitory and bactericidal effects against bacterial isolates from keratitis. SNAC showed greater antimicrobial activity than GSNO against all bacteria. Gram-positive bacteria were more susceptible to the inhibitory and bactericidal effects of the S-nitrosothiols.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Bactericidal Effects of Essential Oils from Clove, Lavender and Geranium on Multi-Drug Resistant Isolates of Pseudomonas aeruginosa

The inhibitory effects of essential oils including clove, lavender and geranium extracted from Eugenia caryophyllata, Lavandula officinalis and Pelargonium graveolens on multidrug resistant isolates of Pseudomonas aeruginosa were investigated. The main constituents of clove, lavander and geranium oil were eugenol (80-90%), 1,8-cineol (13%) and citronellol (45%) respectively. Clove had the most ...

متن کامل

Comparison of Efficacy of Two Disinfectants Against Foodborne Staphylococcus aureus

Background and Objectives: Foodborne illness is a common public health problem. Surfaces and equipment used in food production are involved in the spread of foodborne pathogens. The aim of this study was to evaluate and compare the antimicrobial effect of Vinoxide and Sanisept against Staphylococcus aureus strains isolated from food production lines. Material and Methods: In this descriptive s...

متن کامل

The comparison of antibacterial effect of Schrophularia striata Boiss. and Stachys schtschegleevii Sosn. extracts on pathogens isolated from urinary tract infections

Background & Aim: The present study was aimed to evaluate and compare the antibacterial activity of Schrophularia striata Boiss. andStachys schtschegleevii Sosn. Extracts against seven clinical isolates. The test isolates were Streptococcus agalactiae, Enterococcus faecalis, Staphylococcus saprophyticus, E. coli, Klebsiella pneumoniae, Proteus mirabilis an...

متن کامل

In vitro antibacterial effect of ginger (Zingiber officinale) essential oil against fish pathogenic bacteria isolated from farmed olive flounder (Paralichthys olivaceus) in Korea

The in vitro antibacterial activity of ginger (Zingiber officinale) essential oil (GEO) was tested against five Gram-negative and Gram-positive fish pathgenic bacteria isolated from farmed olive flounder (Paralichthys olivaceus) in Korea. According to the results of disk diffusion, minimum inhibitory and minimum bactericidal concentration (MIC and MBC) tests, GEO was active against most of the ...

متن کامل

Determination of antimicrobial effect of protamine by transmission electron microscopy and SDS PAGE on Pseudomonas aeruginosa isolates from diabetic foot infection

Objective(s): Diabetic foot infection is one of the major complications of diabetes leading to lower limb amputations. Isolation and identification of bacteria causing diabetic foot infection, determination of antibiotic resistance, antimicrobial potential of protamine by electron microscopy and SDS-PAGE analysis, arethe aims of this study.Materials a...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2012